In silico design of short hairpin RNA (shRNA)Molecules for DNA pol gene of Contagious Ecthyma virus (ORFV)

Contagious ecthyma is an infectious skin disease of ruminants caused by the ORF virus (ORFV) that a member genus Parapoxvirus Poxviridae family. In addition to significant effects on lambs and human, ORFVs have been recently shown infect other hosts. The causes economic damages sheep industry, so attempts eliminate it must be taken into.RNA interference (RNAi) evolutionarily conserved mechanism in which expression homologous target genesis suppressed means double-stranded RNA molecules. Since RNAi can considered as therapeutic method for viral gene silencing, we tend make most this capability. present study aims design potential shRNAs knockdown DNA-polymerase coded ORF025.A number computational methods such clustal omega website alignment, BLAST-NCBI similarity search, CLC software secondary structure prediction, BLOCK-iTRNAi Designer WI siRNA Selection Program Software shRNA molecules scoring applied designing against ORF025-DNA pol ORFV. Then three were logically designed gene. conclusion provides strong superior approach achieving validated strategy antiviral molecule meets many sequence features efficient ORFV treatment at mRNA level. efficiency these anti need tested